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Published on: November 11, 2022
[Determination of calceolarioside B in Caulis Akebiae by RP-HPLC]
Hui-Min Gao1, Zhi-Min Wang, Li Qu
1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Objective:
To establish a quantitative method for calceolarioside B in Caulis Akebiae.
Method:
The samples were separated at 35 degrees C on a Phenomenex Luna C18 column eluted with methanol-water-phosphoric acid (35: 65: 0.05) as mobile phase. Flow rate was at 1.0 mL x min(-1) and the detection wavelength was at 330 nm.
Result:
The calibration curve was linear over the range from 0. 04 to 0. 86 g (r = 0. 9998) and the average recovery was 97.2%. 39 Crude drug materials collected from different areas were determined and the contents of calceolarioside B in Caulis Akebiae were fluctuated from 0. 021% to 0. 745%.
Conclusion:
The method is simple and repeatable and could be used for the quality control of Caulis Akebiae.
