Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Correlation between CD154 (CD40L) expression and naive/memory CD4(+) T cells].

Li Li1, Chang-you Wu

  • 1Department of Immunology, School of Preclinical Medicine, Sun Yat-sen University, Guangzhou 510080, China.

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology
|June 8, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Innate Resistance to <i>Leishmania amazonensis</i> Infection in Rat Is Dependent on NOS2.

Frontiers in microbiology·2021
Same author

PD-1<sup>+</sup>CXCR5<sup>-</sup>CD4<sup>+</sup> Th-CXCL13 cell subset drives B cells into tertiary lymphoid structures of nasopharyngeal carcinoma.

Journal for immunotherapy of cancer·2021
Same author

A Novel Hypothesis on Excessive Activation of Residual B Lymphocytes in Common Variable Immunodeficiency Concurrent with Aseptic, Erosive Polyarthritis.

Medical science monitor : international medical journal of experimental and clinical research·2018
Same author

Human memory, but not naive, CD4+ T cells expressing transcription factor T-bet might drive rapid cytokine production.

The Journal of biological chemistry·2014
Same author

Functionally distinct subsets of CD4⁺ regulatory T cells in patients with laryngeal squamous cell carcinoma are indicative of immune deregulation and disease progression.

Oncology reports·2014
Same author

CD45RA-Foxp3high but not CD45RA+Foxp3low suppressive T regulatory cells increased in the peripheral circulation of patients with head and neck squamous cell carcinoma and correlated with tumor progression.

Journal of experimental & clinical cancer research : CR·2014
Same journal

[Research progress on umbilical cord mesenchymal stem cells combined with CAR-NK cells in tumor immunotherapy].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology·2026
Same journal

[Research advances in the molecular mechanisms of microRNA-mediated macrophage lipid homeostasis and foam cell formation].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology·2026
Same journal

[Artificial intelligence-driven assessment of tertiary lymphoid structures in breast cancer: From microscopic analysis to clinical translation].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology·2026
Same journal

[Advances in aberrant crosstalk among key immune cells at the maternal-fetal interface in recurrent spontaneous abortion].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology·2026
Same journal

[The role of innate immune cells in bone metabolism].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology·2026
Same journal

[The dual roles of UFMylation in immune regulation and tumorigenesis].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology·2026
See all related articles

CD154(+) T cells, primarily memory CD4(+) T cells, express key cytokines like IFN-gamma, IL-2, and TNF-alpha. This finding aids in distinguishing naive from memory CD4(+) T cells using CD154 and other markers.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD154 is a crucial molecule in T cell activation and function.
  • Distinguishing between naive and memory T cells is vital for understanding immune responses.

Purpose of the Study:

  • To explore the relationship between CD154 expression on T cells, naive/memory markers, and cytokine production.
  • To determine if CD154 can be used to differentiate naive and memory CD4(+) T cells.

Main Methods:

  • Peripheral blood mononuclear cells (PBMCs) from healthy individuals were analyzed using flow cytometry.
  • Cells were stained for surface markers (CD45RA, CD45RO, CD25, CD62L, CCR7) and intracellular cytokines (IFN-gamma, IL-2, TNF-alpha) after in vitro stimulation.
  • CD154 expression was assessed on T cell subsets.

Related Experiment Videos

Main Results:

  • CD154 was predominantly expressed on CD4(+) T cells, not CD8(+) T cells, after stimulation.
  • The majority of CD4(+) CD154(+) T cells were identified as memory T cells (CD45RO+).
  • CD4(+) CD154(+) T cells produced IFN-gamma, IL-2, and TNF-alpha, with some cells producing multiple cytokines simultaneously.

Conclusions:

  • CD154 expression, in conjunction with other surface markers and cytokine profiles, can effectively discriminate between naive and memory CD4(+) T cells.
  • Short-term in vitro stimulation is sufficient for this analysis.
  • The findings provide a basis for further investigation into the functional roles of CD154(+) T cells.