Related Experiment Video
Updated: Jul 14, 2026

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
Published on: January 31, 2025
Ftm is a novel basal body protein of cilia involved in Shh signalling
Jeanette Vierkotten1, Renate Dildrop, Thomas Peters
1Institut für Entwicklungs- und Molekularbiologie der Tiere EMT, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany.
Abstract:
In this study we show in mice that Ftm (Rpgrip1l) is located at the ciliary basal body. Our data reveal that Ftm is necessary for developmental processes such as the establishment of left-right asymmetry and patterning of the neural tube and the limbs. The loss of Ftm affects the ratio of Gli3 activator to Gli3 repressor, suggesting an involvement of Ftm in Shh signalling. As Ftm is not essential for cilia assembly but for full Shh response, Ftm can be considered as a novel component for cilium-related Hh signalling. Furthermore, the absence of Ftm in arthropods underlines the divergence between vertebrate and Drosophila Hh pathways.
Related Concept Videos
Microtubule Associated Proteins (MAPs)
Microtubule Instability
Microtubules in Signaling
Mechanism of Filopodia Formation
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Assembly of Complex Microtubule Structures
Microtubules in Cell Motility

