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[Principles and application possibilities of cryoultramicrotomy]
S Paradisi1, G Arancia, G Donelli
1Laboratorio di Ultrastrutture, Istituto Superiore di Sanità, Roma.
Annali Dell'Istituto Superiore Di Sanita
|January 1, 1991
Abstract:
This paper reviews the technical aspects of cryoultramicrotomy. The key preparation steps in the technique, namely freezing, sectioning, thawing, and staining are analysed. The application of this method to immunocytochemistry and analytical electron microscopy are discussed with respect to the present possibilities and problems.
