HLA-G alleles and HLA-G 14 bp polymorphisms in a Brazilian population
E C Castelli1, C T Mendes-Junior, E A Donadi
1Division of Clinical Immunology, Department of Medicine, School of Medicine of Ribeirão Preto, University of São Paulo, Avenida Bandeirantes 3900, 14049-900 Ribeirão Preto, São Paulo, Brazil. castelli@usp.br
Abstract:
Brazilians represent one of the most heterogeneous populations in the world as the result of five centuries of interethnic crosses. We investigated the polymorphism of the HLA-G locus in a Brazilian urban sample. HLA-G alleles were determined by SBT analysis of exons 2-4 and 8 in 103 unrelated individuals. The genotyping analysis identified 11 different HLA-G alleles, including the recently described G*010110 allele, and four new variants in this population. These data indicate that this Brazilian population presents high HLA-G allelic polymorphism, suggesting increased levels of intrapopulational genetic diversity that may be because of the admixed nature of the sample. This is the first study regarding HLA-G genotyping that was performed at such refined resolution level in this heterogeneous population.
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