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Updated: Jul 14, 2026
12:11
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
Published on: February 27, 2020
Quantitative analysis of systematic errors originated from wall adsorption and sample plug lengths in affinity
Ning Fang1, Jiangwei Li, Edward S Yeung
1Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA.
Analytical Chemistry
|June 15, 2007
Summary
This study introduces a 2D simulation for capillary electrophoresis, accurately modeling wall adsorption and affinity interactions. The developed model quantifies errors in affinity capillary electrophoresis (ACE) and proposes corrections for binding constant estimation.
Area of Science:
- Analytical Chemistry
- Separation Science
- Biophysical Chemistry
Background:
- Capillary electrophoresis (CE) is a powerful separation technique.
- Affinity capillary electrophoresis (ACE) is used to determine binding constants.
- Wall adsorption can cause peak distortions and errors in ACE.
Purpose of the Study:
- To develop a 2D simulation for capillary electrophoresis.
- To model affinity interactions and wall adsorption simultaneously.
- To quantify errors in ACE binding constant estimation and propose corrections.
Main Methods:
- Developed a 2D simulation using finite difference schemes in cylindrical coordinates.
- Applied a Langmuir second-order kinetic law for wall adsorption/desorption.
- Simulated capillary zone electrophoresis and affinity capillary electrophoresis (ACE).
Main Results:
- Identified parameters for accurate and efficient simulation.
- Quantified peak distortions due to finite sample injection and wall adsorption.
- Demonstrated that finite sample injection and wall adsorption cause systematic errors in ACE binding constants.
Conclusions:
- The 2D simulation accurately models complex CE phenomena.
- Proposed methods can correct binding constant errors in ACE.
- The study enhances the reliability and applicability of ACE.
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