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Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
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Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

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Sensitive bioanalysis: combining single-molecule spectroscopy with mono-labeled self-quenching probes

Nicole Marmé1, Jens-Peter Knemeyer

  • 1Institute of Physical Chemistry, University of Heidelberg, Im Neuenheimer Feld 229, Heidelberg, Germany. marme@single-molecule-spectroscopy.de

Analytical and Bioanalytical Chemistry
|June 15, 2007
PubMed

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...