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Related Experiment Video

Updated: Jul 14, 2026

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation
11:31

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Published on: July 13, 2012

Microbial population dynamics in a thermophilic methane digester fed with garbage.

J Cheon1, F Hong, T Hidaka

  • 1Department of Urban & Environmental Engineering, Kyoto University, Kyoto, Japan. cheonjihoon@biwa.eqc.kyoto-u.ac.jp

Water Science and Technology : a Journal of the International Association on Water Pollution Research
|June 15, 2007
PubMed
Summary

This study investigated microbial diversity in thermophilic anaerobic digesters treating garbage, sewage sludge, and livestock waste. Bacillus infernus and Methanothermobacter wolfeii were identified as key microbes in garbage digesters.

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Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
06:17

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

Published on: August 15, 2019

Area of Science:

  • Microbiology
  • Environmental Science
  • Biotechnology

Background:

  • Thermophilic anaerobic digestion is crucial for waste management.
  • Understanding microbial communities is key to optimizing digester efficiency.
  • Previous studies have not fully characterized microbial dynamics in diverse waste streams.

Purpose of the Study:

  • To investigate microbial diversity in three full-scale thermophilic anaerobic digesters (TGD, TSD, TLD).
  • To study population dynamics in a thermophilic garbage digester (TGD).
  • To identify key microbes and assess method reproducibility in thermophilic methane digesters.

Main Methods:

  • 16S rDNA clone libraries were used for microbial community analysis.
  • Triplicate analyses were performed for each digester type.
  • Population dynamics were monitored over 90 days using clone libraries.

Main Results:

  • 736 clones were identified, with an estimated error of +/-10% for OTUs.
  • The most frequent OTUs were related to Uncultured bacterium MBA08 (TGD), Unidentified bacterium TUG22 (TSD), and Uncultured archaeal symbiont PA204 (TLD).
  • Bacillus infernus and Methanothermobacter wolfeii populations in TGD fluctuated with VFA concentration.

Conclusions:

  • Bacillus infernus and Methanothermobacter wolfeii are identified as key functional microbes in thermophilic garbage digesters.
  • The study provides insights into microbial diversity and population dynamics in anaerobic digestion.
  • The findings contribute to optimizing thermophilic methane production from waste materials.