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Related Experiment Video

Updated: Jul 8, 2026

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
08:14

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

Published on: May 1, 2014

An integrated gene regulatory network controls stem cell proliferation in teeth.

Xiu-Ping Wang1, Marika Suomalainen, Szabolcs Felszeghy

  • 1Developmental Biology Programme, Institute of Biotechnology, Viikki Biocenter, University of Helsinki, Finland.

Plos Biology
|June 15, 2007
PubMed
Summary

Stem cell niches regulate tissue regeneration. In mouse incisors, Activin, BMP, FGF, and Follistatin signaling control stem cell proliferation and generate asymmetry, revealing insights into organogenesis.

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Last Updated: Jul 8, 2026

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
08:14

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Published on: May 1, 2014

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Genetics

Background:

  • Epithelial stem cells in niches drive tissue regeneration (hair, skin, gut).
  • Mammalian teeth have limited regeneration, but mouse incisors continuously grow, featuring stem cells in cervical loops.
  • Labial cervical loops have proliferative stem cells forming enamel, while lingual loops have fewer stem cells and lack enamel.

Purpose of the Study:

  • Identify signaling molecules regulating rodent incisor stem cell proliferation.
  • Elucidate the role of these molecules in generating incisor asymmetry.

Main Methods:

  • Mouse mutant analyses
  • Organ culture experiments
  • Gene expression studies

Main Results:

  • Epithelial stem cell proliferation is controlled by Activin, BMP, FGF, and Follistatin.
  • Mesenchymal FGF3 stimulates proliferation; BMP4 represses Fgf3.
  • Activin inhibits BMP4, restricting Fgf3 to labial mesenchyme, increasing labial stem cell proliferation and niche size.
  • Follistatin limits lingual stem cells, contributing to incisor asymmetry.

Conclusions:

  • A gene regulatory network balances signaling to control stem cell proliferation and asymmetric organogenesis in incisors.
  • This network involves Activin, BMP, FGF, and Follistatin.
  • Variations in such networks may explain differing regenerative capacities across organs and species.