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Radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol
Published on: September 21, 2021
Purification of [18F]-fluoro-L-thymidine ([18F]-FLT) for positron emission tomography imaging
Mickaël Bourgeois1, Marie Mougin-Degraef1, Françoise Leost2
1Département de Recherche en Cancérologie, Université de Nantes, INSERM, U601, Institut de Biologie, 9 quai Moncousu, 44093 Nantes Cedex 1, France.
Abstract:
3'-Deoxy-3'-[18F]fluorothymidine ([18F]-FLT) has recently been described as a positron emission tomography (PET) radiopharmaceutical for visualizing cellular proliferation in vivo. All published radiosyntheses of [18F]-FLT provide crude products that must be purified before injection to human. This study describes a reliable purification procedure for [18F]-FLT. It is based on HPLC. In 17.9+/-0.5 min, pure [18F]-FLT is obtained that could be injected to human after a passage through a sterile 0.22 microm filter.
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