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Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation

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Published on: November 17, 2017

Conditional targeting: inducible deletion by Cre recombinase.

Kelly R O'Neal¹, Ramtin Agah

¹The Program in Human Molecular Biology and Genetics, University of Utah, Salt Lake City, USA.

Methods in Molecular Biology (Clifton, N.J.)

|June 15, 2007

Summary

This study details a method for tissue-specific gene knockouts in mice using Cre recombinase. This technique allows for precise control over gene deletion in cardiovascular disease models.

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Area of Science:

Cardiovascular Research
Genetics
Molecular Biology

Background:

Cre recombinase technology offers versatile temporal and spatial control for gene deletion.
Generating genetically modified mouse models is crucial for studying complex diseases like cardiovascular disease.

Purpose of the Study:

To describe a method for inducible, tissue-specific gene knockout in mice.
To enable precise investigation of gene function in cardiovascular disease models.

Main Methods:

Utilizing Cre-loxP system for targeted gene deletion.
Generating double transgenic mouse lines with inducible, tissue-specific Cre expression.
Crossing these lines with mice carrying floxed genes of interest.
Administering an exogenous agent to induce gene knockout at a specific time and tissue.

Main Results:

Successful generation of mice with inducible, cardiac-specific gene knockout.
Demonstrated experimental versatility of the Cre system for gene deletion.
Established a protocol for precise genetic manipulation in disease modeling.

Conclusions:

The described Cre-loxP system provides a powerful tool for cardiovascular research.
This method allows for detailed study of gene function in specific tissues.
Inducible, tissue-specific gene knockouts enhance the utility of murine disease models.