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Gene expression in pyriproxyfen-resistant Bemisia tabaci Q biotype.

Murad Ghanim1, Svetlana Kontsedalov

  • 1Institute of Plant Protection, Department of Entomology, Volcani Centre, Bet Dagan 50250, Israel. ghanim@agri.gov.il

Pest Management Science
|June 15, 2007
PubMed
Summary

Pyriproxyfen resistance in whiteflies is increasing. This study identified 111 upregulated genes in resistant Bemisia tabaci, offering insights into pyriproxyfen

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Area of Science:

  • Entomology
  • Molecular Biology
  • Pest Management

Background:

  • Pyriproxyfen is a key insecticide for Bemisia tabaci control in integrated pest management (IPM).
  • Insecticide resistance, particularly to pyriproxyfen, is a growing problem for whitefly control.
  • The molecular mechanisms underlying pyriproxyfen resistance remain largely unknown.

Purpose of the Study:

  • To investigate the molecular basis of pyriproxyfen resistance in Bemisia tabaci.
  • To identify genes and pathways involved in pyriproxyfen resistance.
  • To lay the groundwork for developing molecular markers for resistance monitoring.

Main Methods:

  • Utilized a cDNA microarray to analyze gene expression changes in a pyriproxyfen-resistant Bemisia tabaci strain.

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  • Compared gene expression profiles between resistant and susceptible whitefly populations after pyriproxyfen exposure.
  • Performed statistical analysis to identify differentially expressed genes (expressed sequence tags - ESTs).

    • Main Results:

    • Identified 111 expressed sequence tags (ESTs) that were significantly upregulated in the resistant strain post-treatment.
    • Upregulated ESTs included genes associated with known resistance mechanisms like P450s, oxidative stress, and mitochondrial function.
    • Observed upregulation of genes related to metabolism (protein, lipid, carbohydrate) and juvenile hormone (JH)-associated processes, including oocyte and egg development.

    Conclusions:

    • The study reveals significant changes in gene expression associated with pyriproxyfen resistance in Bemisia tabaci.
    • Upregulated genes suggest a complex resistance mechanism involving detoxification, metabolic adaptation, and potentially altered JH signaling.
    • Findings provide a foundation for understanding pyriproxyfen's mode of action and developing resistance management strategies.