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Updated: Jul 14, 2026

Isolating Human Peripheral Blood Mononuclear Cells from Buffy Coats via High Throughput Immunomagnetic Bead Separation
Published on: July 19, 2024
Comparison of blood component preparation methods from whole blood bags based on buffy coat extraction
Joan Cid1, Montse Claparols, Asunción Pinacho
1Banc de Sang i Teixits, Passeig Vall d'Hebron, 119-129, 08035 Barcelona, Spain. jcid@vhebron.net
Abstract:
We compared the data of our quality control laboratory of the blood components according to the blood component preparation method that we used. We prepared blood components from top and top whole blood (WB) bags and manual pooling of buffy coats (BCs) (method I) or from top and bottom WB bags and automated pooling of BCs with OrbiSac (method II). Pooled platelet concentrates (PC) obtained with method II had higher platelet content when compared with pooled PCs obtained with method I (3.5+/-0.7x10(11) vs. 2.6+/-0.8x10(11); p<0.001). The hemoglobin content in the RBCs obtained with method I was higher when compared with method II (55+/-7g vs. 52.5+/-6.6g; p<0.001).

