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[Anti-oxidative effect of Angelica polysaccharide sulphate]
Min Jia1, Tie-hong Yang, Xiu-juan Yao
1Department of Pharmacology, Fourth Military Medical University, Xi'an 710032, China.
Objective:
To investigate the anti-oxidative effect of Angelica polysaccharide sulphate( APS).
Methods:
The Hela cells were cultured conventionally. Then APS was added and cultured together in different concentration for 24h followed by a oxidative injury with H2O2 or UV irradiation. The anti-oxidative effects of APS were detected as follow: cell viability was measured by MTT assay; colorimetric analysis was used to determine SOD activity, GSH and MDA level in cytoplasm.
Results:
Treatment of H2O2 or UV irradiation significantly decreased cell viability, GSH and SOD activity in cytoplasm,while increased MDA in cytoplasm. At the range of 0. 3 -100microg/ml, APS significantly increased cell viabilty, GSH and SOD activity,while decreased MDA in a dose dependent manner( P < 0. 05 or P <0.01).
Conclusion:
APS has anti-oxidative effect,which may be one of its anti-AIDS mechanisms.