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Updated: Jul 14, 2026

OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
Published on: June 20, 2010
[Study on the extraction process of polysaccharide from Agrocybe cylindracea]
Hui-ling Mao1, Ying-hui Shu, Wen Yang
1The College of Bioligical Secience, Nanchang University, Nanchang 330031, China.
Objective:
to study the optimum extracting Process of Agrocybe cylindracea polysaccharides.
Methods:
The technique conditions of Agrocybe cylindracea polysaccharides aere opeimized by the hot water extraction. determined the content of polysaccharides as the estimative targets and choosed the single-factor experiment and the orthogonal design of L1, (35 ) to investgate the polysaccharides content influenced by five factors such as water sample ratio, pH value, extraction temperature, ethanol concentration, the time of extraction.
Results:
The optimum extraction conditions uere as follows: water sample ratio 1: 10, pH7. 5 , extaction temperature was 90 degrees C , one hour every time,ethanol concentration was 95%. made the experiment foundation for optimizing the extraction methods of Agrocybe cylindracea polysaccharides.

