Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Surface Active Agents01:27

Surface Active Agents

Surfactants, named for their behavior at interfaces, positively adsorb at the interfaces of two phases, reducing interfacial tension. Their versatility as emulsifiers, detergents, and foaming agents stems from this ability. Surfactants, often termed amphiphiles, share the property of amphipathy, with molecules having both hydrophilic and hydrophobic portions. The hydrophilic part is called the head, and the hydrophobic part, including an elongated alkyl substituent, forms the tail.Surfactants...
Micelles01:30

Micelles

Micelle formation is an intricate process that hinges on the properties of amphiphilic or amphipathic molecules and the conditions of the system in which they are found. Amphiphilic molecules, which have both hydrophilic (water-attracting) and hydrophobic (water-repelling) parts, play a critical role in this process.In aqueous environments, these molecules arrange themselves such that their hydrophilic heads are turned towards the water phase, while their hydrophobic tails are oriented away...
Solubility03:00

Solubility

Solution, Solubility, and Solubility Equilibrium
A solution is a homogeneous mixture composed of a solvent, the major component, and a solute, the minor component. The physical state of a solution—solid, liquid, or gas—is typically the same as that of the solvent. Solute concentrations are often described with qualitative terms such as dilute (of relatively low concentration) and concentrated (of relatively high concentration).
In a solution, the solute particles (molecules, atoms, and/or ions)...
Halogens03:01

Halogens

Group 17 elements, known as halogens, are nonmetals. At room temperature, fluorine and chlorine are gases, bromine is a liquid, and iodine a solid. Astatine is a highly unstable radioactive element, so currently, most of its properties are unknown due to its short half-life. Tennessine is a synthetic element also predicted to be in this group.
Colloids03:22

Colloids

Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...
Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biobased chitosan films incorporating rosemary-mediated silver nanoparticles for enhanced antimicrobial activity and catalytic applications.

International journal of biological macromolecules·2026
Same author

Pre-adsorption of serum albumin on biomaterial surfaces modulates bacteria-surface interactions and alters bacterial physiological responses.

Materials today. Bio·2026
Same author

Bioinspired and Biomimetic Wetting Properties.

ACS nano·2025
Same author

Advances in immune mechanisms and developing immune-targeted therapies for osteoporosis: A systematic review.

Pharmacological research·2025
Same author

Hybrid structure combining essential oil derivatives and polydopamine for anti-bacterial coatings.

Colloids and surfaces. B, Biointerfaces·2025
Same author

Soft-Template Electropolymerization from Triphenylamine-Based Monomers: From Vertically Aligned Nanotubes to Nanomembranes.

ChemistryOpen·2025

Related Experiment Video

Updated: Jul 14, 2026

Studying Surfactant Effects on Hydrate Crystallization at Oil-Water Interfaces Using a Low-Cost Integrated Modular Peltier Device
06:31

Studying Surfactant Effects on Hydrate Crystallization at Oil-Water Interfaces Using a Low-Cost Integrated Modular Peltier Device

Published on: March 18, 2020

2-DE using hemi-fluorinated surfactants.

Mireille Starita-Geribaldi1, Pascal Thebault, Elisabeth Taffin de Givenchy

  • 1Connexines et Prolifération Germinale: Physiopathologie Cellulaire et Moléculaire (INSERM U670), UFR de Médecine, Université de Nice Sophia-Antipolis, Nice, France. mgeribal@unice.fr

Electrophoresis
|June 20, 2007
PubMed
Summary

New hemi-fluorinated zwitterionic surfactants (FASB-p,m) enhance proteomic analysis by improving two-dimensional electrophoresis (2-DE) separation. These surfactants selectively solubilize membranes, aiding in protein extraction and characterization.

More Related Videos

Enhanced Oil Recovery using a Combination of Biosurfactants
13:19

Enhanced Oil Recovery using a Combination of Biosurfactants

Published on: June 3, 2022

Extraction and Characterization of Surfactants from Atmospheric Aerosols
09:34

Extraction and Characterization of Surfactants from Atmospheric Aerosols

Published on: April 21, 2017

Related Experiment Videos

Last Updated: Jul 14, 2026

Studying Surfactant Effects on Hydrate Crystallization at Oil-Water Interfaces Using a Low-Cost Integrated Modular Peltier Device
06:31

Studying Surfactant Effects on Hydrate Crystallization at Oil-Water Interfaces Using a Low-Cost Integrated Modular Peltier Device

Published on: March 18, 2020

Enhanced Oil Recovery using a Combination of Biosurfactants
13:19

Enhanced Oil Recovery using a Combination of Biosurfactants

Published on: June 3, 2022

Extraction and Characterization of Surfactants from Atmospheric Aerosols
09:34

Extraction and Characterization of Surfactants from Atmospheric Aerosols

Published on: April 21, 2017

Area of Science:

  • Biochemistry
  • Proteomics
  • Analytical Chemistry

Background:

  • Two-dimensional electrophoresis (2-DE) is crucial for proteomic analysis, but protein solubilization remains a challenge.
  • Zwitterionic surfactants are commonly used, but their efficacy can be limited for hydrophobic proteins.
  • Fluorinated surfactants offer unique properties that may improve solubilization and separation.

Purpose of the Study:

  • To synthesize and evaluate novel hemi-fluorinated amidosulfobetaine (FASB-p,m) surfactants for 2-DE.
  • To compare the performance of FASB-p,m surfactants with their hydrocarbon counterparts (ASB-n).
  • To investigate the effect of FASB-p,m on red blood cell (RBC) membrane protein solubilization.

Main Methods:

  • Synthesis of hemi-fluorinated amidosulfobetaine (FASB-p,m) surfactants.
  • Comparison with hydrocarbon amidosulfobetaine (ASB-n) surfactants.
  • Application in 2-DE for proteomic analysis.
  • Assessment of red blood cell (RBC) membrane protein solubilization.

Main Results:

  • FASB-p,m surfactants demonstrated modulated lipophilicity due to partial fluorination.
  • Specific solubilization of RBC membrane components was observed, dependent on hydrophobic chain length.
  • Increased number of polypeptide spots in 2-DE patterns when using FASB-p,m.
  • The oleophobic nature of FASB-p,m prevented solubilization of the hydrophobic transmembrane protein Band 3.
  • Band 3 was successfully solubilized in a subsequent step using ASB-14.

Conclusions:

  • Hemi-fluorinated zwitterionic surfactants (FASB-p,m) are effective tools for enhancing 2-DE in proteomic analysis.
  • These surfactants facilitate a two-step solubilization strategy, improving the extraction of challenging proteins like Band 3.
  • FASB-p,m surfactants offer a novel approach to membrane protein solubilization and analysis.