Related Experiment Video
Updated: Jul 14, 2026
Human Blastocyst Biopsy and Vitrification
Published on: July 26, 2019
Vitrification of human hatched blastocysts: a report of 4 cases
Kenichiro Hiraoka1, Kaori Hiraoka, Masayuki Kinutani
1Kinutani Women's Clinic, 2-1-4-3F, Ohtemachi, Naka-ku, Hiroshima 730-0051, Japan. hiraoka@chive.ocn.ne.jp
Background:
Vitrification is a fairly well-established technique for cryopreservation of human zona-intact blastocysts. However, little is known about the efficacy of the vitrification technique for zona-free hatched blastocysts.
Case:
A total of 4 hatched blastocysts from 4 healthy, infertile women undergoing in vitro fertilization were vitrified and warmed. All 4 hatched blastocysts expanded after warming and were transferred to 4 patients. Three of the women became clinically pregnant. Two healthy infants were born in 2 deliveries, and 1 pregnancy had progressed to 35 weeks at this writing.
Conclusion:
Our results suggest that vitrification is a useful cryopreservation technique not only for zona-intact blastocysts but also for zona-free hatched blastocysts.
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08:28Morphometric Protocol for the Objective Assessment of Blastocyst Behavior During Vitrification and Warming Steps
Published on: February 28, 2019
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