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Updated: Jul 14, 2026

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
Published on: May 14, 2020
The Arabidopsis nitrate transporter AtNRT2.1 is targeted to the root plasma membrane
Franck Chopin1, Judith Wirth, Marie-France Dorbe
1INRA, IJPB, Unité de Nutrition Azotée des Plantes, Route de St Cyr, 78026 Versailles cedex, France.
Abstract:
Arabidopsis AtNRT2.1 protein is the best characterized high affinity nitrate transporter in higher plants. However, nothing is known about its sub-cellular localization. In this work, we used GFP imaging to follow the targeting of the AtNRT2.1 protein to the different cell membranes. A polyclonal antibody was also raised against a peptide derived from the AtNRT2.1 sequence. Comparison of wild type and mutant plant extracts showed that this antibody recognized specifically the AtNRT2.1 protein. Microsomal membranes were fractionated on sucrose gradients and immunological detections were performed on the different fractions. Altogether, our results demonstrate that the AtNRT2.1 protein is located in the plasma membrane of the root cells.
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