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Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
Published on: March 23, 2020
Charge switch derivatization of phosphopeptides for enhanced surface-enhanced Raman spectroscopy and mass
Handong Li1, Narayan Sundararajan
1Biomedical and Life Sciences, Digital Health Group, Intel Corporation, Santa Clara, California 95054, USA.
Journal of Proteome Research
|June 23, 2007
Abstract:
We report an aqueous one-pot reaction chemistry to derivatize phosphopeptides by switching the negatively charged phosphate group to a positively charged phosphonium or ammonium moiety. The phosphonium or ammonium tagged peptides then serve as peptide or protein phosphorylation signatures allowing extended and more sensitive analyses using surface-enhanced Raman spectroscopy (SERS) and mass spectrometry.

