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Updated: Jul 14, 2026
Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
Published on: January 17, 2020
Determination of chromium(VI) by surface plasmon field-enhanced resonance light scattering
Zhiqiang Han1, Li Qi, Gangyi Shen
1Beijing National Laboratory of Molecular Science, Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080, People's Republic of China.
Abstract:
A surface plasmon-enhanced resonance light scattering method has been developed. The method features strong light scattering but very weak background, and after incorporating with selective sample extraction and ion-association complexation using rhodamine B and KI as reactants, it could selectively determine Cr(VI) in both of standard and real samples, reaching a limit of detection down to 20 nM which is about 40-fold as sensitive as flame atomic absorption spectrometry and 140-fold as sensitive as fluorescent spectroscopy. Its linear working range was found in between 40 and 320 nM, with a relative standard deviation of peak height at <3% (n = 5) and recovery between 94.8-104.9%. In theory, the method is applicable to the analysis of all substances able to produce or destroy I2.
