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Updated: Jul 14, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
Published on: September 19, 2018
Capillary electrophoresis as a second dimension to isoelectric focusing for peptide separation
Jean-Marc Busnel1, Niels Lion, Hubert H Girault
1Laboratoire d'Electrochimie Physique et Analytique, Station 6, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
Abstract:
Capillary zone electrophoresis and carrier ampholytes based capillary electrophoresis have been used as a second separation step to Off-Gel isoelectric focusing for the analysis of complex peptide mixtures. A tryptic digest of four proteins (bovine serum albumin, beta-lactoglobulin, horse myoglobin, cytochrome c) has been chosen as a peptide test mixture. After assessment of different modes of capillary electrophoresis as a second dimension to Off-Gel isoelectric focusing, the optimized two-dimensional platforms provide a degree of orthogonality comparable to state-of-the-art multidimensional liquid chromatography systems as well as a practical peak capacity above 700.
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