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Related Concept Videos

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Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products
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Tracing Salmonella in Alheira processing plants.

A Esteves1, T Aymerich, M Garriga

  • 1Department of Veterinary Sciences, Center of Studies in Animal and Veterinary Sciences CECAV, University of Trás-os Montes e Alto Douro, Vila Real, Portugal. alexe@utad.pt

Journal of Applied Microbiology
|June 23, 2007
PubMed
Summary

Contaminated casings and surfaces are the primary sources of Salmonella spp. contamination in Alheira. Polymerase chain reaction (PCR) molecular typing confirmed cross-contamination, aiding pathogen control strategies.

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Area of Science:

  • Food microbiology
  • Food safety
  • Veterinary public health

Background:

  • Salmonella spp. is a significant foodborne pathogen.
  • Alheira production involves complex processing steps.
  • Identifying contamination sources is crucial for public health.

Purpose of the Study:

  • Investigate Salmonella spp. sources in Alheira production.
  • Determine Salmonella spp. distribution across production lines.
  • Apply multifactorial correspondence analysis and PCR for tracing.

Main Methods:

  • Analysis of four production lines, four Alheira batches, and 14 sampling sites.
  • Microbial analysis of 896 samples, yielding 85 Salmonella spp. isolates.
  • Utilized multifactorial correspondence analysis and PCR molecular typing.

Main Results:

  • Salmonella spp. presence in Alheira directly linked to contaminated casings.
  • PCR molecular typing identified cross-path contamination from surfaces.
  • Occurrence data analysis confirmed casings as the primary contamination source.

Conclusions:

  • Contaminated casings and surfaces are key sources of Salmonella spp. in Alheira.
  • PCR molecular typing offers high discriminatory power for tracing contamination.
  • Understanding contamination pathways enables improved pathogen control strategies.