Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tissue-specific mRNA expression profiling in grape berry tissues.

Jerome Grimplet1, Laurent G Deluc, Richard L Tillett

  • 1Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557, USA. grimplet@unr.edu <grimplet@unr.edu>

BMC Genomics
|June 23, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Overexpression of cytosolic NADP-malic enzyme 1 from the common ice plant enhances water-deficit and high-light stress tolerance by modulating water-use efficiency and flavonoid biosynthesis.

The Plant journal : for cell and molecular biology·2026
Same author

Optimal Methods for Estimating Cactus Pear Biomass Using Cladode Dimensions of Morphologically Diverse Accessions.

Plant, cell & environment·2026
Same author

Revisiting tandem duplication in plant genomes: Technical challenges and pangenome solutions.

Current opinion in plant biology·2026
Same author

Assessing completeness of cancer family history in EHRs using genetically defined kinships: A cross-sectional study.

Genetics in medicine open·2025
Same author

Synthetic crassulacean acid metabolism (SynCAM) for improving water-use efficiency in plants.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2025
Same author

Early Activation of RNAi Reveals Genomic Regions of Grapevine Red Blotch Virus Targeted for Silencing in Grapevine.

Molecular plant-microbe interactions : MPMI·2025
Same journal

Genome-wide analysis across Indian camel populations reveals genetic distinctiveness of the Kharai camel breed.

BMC genomics·2026
Same journal

Different genomic footprint of small insertion-deletion and structural variants determines the genetic divergence of indica and japonica rice.

BMC genomics·2026
Same journal

From nurse bee to queen egg: RNA-seq analysis of Apis mellifera eggs shows dietary protein-dependent gene regulation.

BMC genomics·2026
Same journal

A genome-wide association study to identify the genetic loci underlying carbapenem resistance in Acinetobacter baumannii.

BMC genomics·2026
Same journal

Comparative transcriptome analysis to reveal key drought stress-responsive genes in sorghum (Sorghum bicolor (L.) Moench).

BMC genomics·2026
Same journal

Tissue identity is the dominant determinant of cross-species transferability of a porcine developmental programme.

BMC genomics·2026
See all related articles

Grape berry tissues (skin, pulp, seed) show distinct gene expression patterns crucial for wine quality. Water-deficit stress significantly alters these patterns, impacting aroma and color metabolite biosynthesis.

Area of Science:

  • Plant Biology
  • Genomics
  • Viticulture

Background:

  • Grape berries (Vitis vinifera) comprise skin, pulp, and seed, each contributing unique compounds to wine's aroma, color, and flavor.
  • These tissues protect and aid seed dispersal, with skin providing aroma/color compounds, pulp sugars/acids, and seeds tannins.
  • Understanding tissue-specific gene expression is key to grape berry development and wine quality.

Purpose of the Study:

  • To profile mRNA expression across grape berry tissues (skin, pulp, seed).
  • To investigate the influence of water-deficit stress on tissue-specific gene expression.
  • To identify transcriptional networks governing tissue differentiation and stress responses.

Main Methods:

  • Utilized Affymetrix GeneChip Vitis oligonucleotide microarray for mRNA expression profiling.

Related Experiment Videos

  • Analyzed gene expression in mature Cabernet Sauvignon grape berries from well-watered and water-deficit stressed vines.
  • Compared expression patterns across skin, pulp, and seed tissues.

    • Main Results:

    • Berry tissues express approximately 76% of genes on the microarray, with 60% showing differential expression.
    • Significant differences in gene expression were observed between seed, pulp, and skin tissues.
    • Water-deficit stress affected 13% of genes, primarily in pulp and skin, altering aroma and color metabolite biosynthesis.

    Conclusions:

    • Revealed novel insights into extensive tissue-specific mRNA expression patterns in grape berries.
    • Established a comprehensive gene expression catalog for future research on transcriptional regulation.
    • Confirmed water-deficit stress profoundly impacts mRNA expression, affecting key metabolites and ultimately wine quality.