Optical tweezers: tethers, wavelengths, and heat

This chapter briefly review the four major methods of optical trapping: (1) directly on to single cells or groups of cells, (2) directly on to organelles and structures inside of the cell, (3) on to a bead as a "handle" to apply force, and (4) on to a bead that has been coated with an antigen or antibody that is moved to the cell membrane for the purpose of activation of a chemical response (no force is applied to the cell). In addition, this chapter discusses the issue of optimal wavelength selection for trapping and the potential temperature rise within the trap.