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Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging
Published on: July 28, 2011
Automated motile cell capture and analysis with optical traps
Bing Shao1, Jaclyn M Nascimento, Linda Z Shi
1Department of Electrical and Computer Engineering, University of California, San Diego, La Jolla, California 92093, USA.
Methods in Cell Biology
|June 26, 2007
Summary
Optical trapping quantifies sperm motility by measuring swimming forces using a laser trap. This technology enables precise semen quality assessment and automated sperm analysis.
Area of Science:
- Biomedical Engineering
- Biophysics
- Reproductive Biology
Background:
- Laser trapping is a noninvasive, microfluidic-compatible tool for biomedical applications.
- Sperm motility is a critical factor in semen quality and reproductive success.
Purpose of the Study:
- To utilize optical trapping for quantitative measurement of sperm motility.
- To assess sperm swimming forces as an indicator of semen quality.
Main Methods:
- Employed a single point gradient laser trap to measure sperm swimming forces.
- Developed custom software, including the single sperm tracking algorithm (SSTA) and a real-time automated tracking and trapping system (RATTS).
- Utilized an annular laser trap for potential high-throughput sperm sorting.
Main Results:
- Direct measurement of sperm swimming forces provides quantitative data on motility.
- Automated systems (RATTS) minimize human intervention for efficient sperm analysis.
- Developed an annular trap for gentle study of sperm behavior and potential sorting.
Conclusions:
- Optical trapping offers a precise method for evaluating sperm motility and semen quality.
- Automated tracking and trapping systems enhance experimental efficiency and reduce operator dependence.
- Novel laser trap designs facilitate advanced applications like high-throughput sperm sorting.
