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Related Experiment Videos

Multilayer PDMS microfluidic chamber for controlling brain slice microenvironment.

A J Blake1, T M Pearce, N S Rao

  • 1University of Wisconsin-Madison, Department of Biomedical Engineering, 1550 Engineering Drive, Madison, WI 53706, USA.

Lab on a Chip
|June 28, 2007
PubMed
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Researchers developed a novel microfluidic device for brain slice experiments. This device maintains slice viability and allows precise, localized control of perfusate flow for advanced neurophysiological studies.

Area of Science:

  • Neuroscience
  • Bioengineering
  • Physiology

Background:

  • Maintaining brain slice viability and precise control over the microenvironment is crucial for studying neural activity.
  • Traditional methods often lack the spatiotemporal resolution needed for targeted solution delivery.
  • Developing advanced tools is essential for understanding complex neural circuits and functions.

Purpose of the Study:

  • To develop and characterize a novel three-layer microfluidic polydimethylsiloxane (PDMS) device for maintaining and perfusing brain slices.
  • To demonstrate the device's capability for precise, localized delivery of solutions to specific regions of a brain slice.
  • To assess the device's suitability for long-term electrophysiological recordings and neurophysiological studies.

Main Methods:

Related Experiment Videos

  • Fabrication of a three-layer PDMS microfluidic device with two fluid chambers and microposts.
  • Perfusion of neonatal rat medullary brain slices (530-700 microm thickness) within the device.
  • Application of laminar perfusate flow and targeted delivery of Na(+)-free solutions to specific slice regions.
  • Monitoring of spontaneous, rhythmic, respiratory-related motor output and neural activity.

    • Main Results:

    • The microfluidic device successfully maintained brain slice viability for up to 3 hours, producing rhythmic motor output.
    • Independent control of perfusate flow in separate chambers allowed for precise, localized solution delivery.
    • Targeted application of a Na(+)-free solution abolished neural activity in one half of the slice while the other remained active.
    • Demonstrated ability to focus different solutions over the midline of the brain slice.

    Conclusions:

    • The novel multilayer microfluidic chamber enables long-term maintenance and perfusion of brain slices.
    • The device offers high spatiotemporal resolution for controlled drug or solution delivery, crucial for neurophysiological research.
    • This technology integrates well with standard electrophysiology tools, advancing studies on neural circuits and drug efficacy.