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Published on: May 20, 2013
The role of UHPLC in pharmaceutical development
Stephen M Chesnut1, John J Salisbury
1Analytical Research and Development, Pfizer Global Research and Development, Groton/New London Laboratories, Groton, CT 06340, USA.
Journal of Separation Science
|June 29, 2007
Summary
Ultra-high-performance liquid chromatography (UHPLC) offers significant advantages for pharmaceutical separations, decreasing run times up to sevenfold and enhancing efficiency for high-resolution applications compared to traditional HPLC.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Science
Background:
- Traditional High-Performance Liquid Chromatography (HPLC) has been the standard for pharmaceutical separations.
- Emerging Ultra-High-Performance Liquid Chromatography (UHPLC) utilizes sub-2 micrometer particle size columns and increased linear velocities.
- UHPLC systems can operate at pressures exceeding 400 bar, requiring mobile phase viscosity management or system redesign.
Purpose of the Study:
- To evaluate the impact and potential roles of UHPLC in pharmaceutical analysis.
- To compare the performance of UHPLC against conventional HPLC for various separation goals.
Main Methods:
- UHPLC employs sub-2 micrometer particle size columns.
- Increased linear velocities are utilized in UHPLC.
- Research involved comparative analysis against traditional HPLC methods.
Main Results:
- UHPLC can decrease pharmaceutical separation run times by up to 7x.
- UHPLC demonstrates significant efficiency advantages for high-resolution applications.
- UHPLC offers increased speed without sacrificing chromatographic efficiency.
Conclusions:
- UHPLC presents a powerful advancement for pharmaceutical method development and analysis.
- The technology enables faster and more efficient separations, particularly for complex or high-resolution tasks.
- Pharmaceutical chemists should consider adopting UHPLC for improved analytical outcomes.
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