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Updated: Apr 16, 2026
Light-driven Enzymatic Decarboxylation
Published on: May 22, 2016
Method development and validation of arene substituted regioisomers in a pharmaceutical candidate by high temperature
John J Salisbury1, Nga M Do2, John A Ragan2
1Analytical Research and Development, Pfizer Worldwide Research and Development, Eastern Point Road, Groton, CT 06340, USA.
Abstract:
This paper describes the development and validation of a high temperature gas chromatography flame ionization detection (HTGC-FID) method for the purity evaluation of arene substituted regioisomers in a key starting material of a pharmaceutical candidate in Phase 3 studies. The chromatographic conditions of the method employ a (5%-phenyl)-methylpolysiloxane packed column (30m×0.25mm) at a constant flow of 1.0mLmin(-1) with a gradient temperature program from 150°C to 400°C with injector and detector temperatures of 300°C and 340°C, respectively. The calibration curve for the desired product (r=0.9999) was assessed for five points in the range from approximately 1.0μgmL(-1) to 40μgmL(-1). The precision (% RSD) of the method was calculated for six replicate injections and found to be 0.81%. The limits of detection and quantitation were determined to be 0.06 and 0.20μgmL(-1), respectively.
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