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Updated: Aug 21, 2026

Ganglioside Extraction, Purification and Profiling
Published on: March 12, 2021
Analysis of Ganglioside Molecular Species Focusing on Ceramide Structures Using Thin-Layer Chromatography-Mass
Noboru Hashimoto1,2, Shizuka Ito3, Akihito Yamamoto4
1Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya, Japan. hashimoto.noboru.t3@f.mail.nagoya-u.ac.jp.
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Thin-layer chromatography is widely used for the analysis of glycolipids and phospholipids. Typically, after separation with various solvents, molecular species are identified by chemical detection or immunostaining. This chapter describes a method for structural analysis of molecules by directly performing mass spectrometry after chromatographic separation. We introduce a classical ganglioside analysis method and a technique for structure analysis of separated lipids using a robotic ion source for direct MS analysis.

