Related Experiment Video
Updated: Jul 14, 2026

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms
Published on: December 1, 2014
Use of quantitative PCR and culture methods to characterize ecological flux in bacterial biofilms
F Dalwai1, D A Spratt, J Pratten
1Division of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London, United Kingdom. f.dalwai@eastman.ucl.ac.uk
Abstract:
An in vitro model of supragingival plaque associated with gingivitis was characterized by traditional culture techniques, comparative 16S rRNA gene sequencing of isolates, and quantitative PCR (QPCR). Actinomyces naeslundii, Prevotella spp., and Porphyromonas gingivalis increased under conditions emulating gingivitis. Gram-negative species and total bacteria were dramatically underestimated by culture compared to the estimates obtained by QPCR.

