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Updated: Jul 14, 2026
Cell-Free DNA Extraction of Vitreous and Aqueous Humor Specimens for Diagnosis and Monitoring of Vitreoretinal Lymphoma
Published on: January 12, 2024
Vitreous humour as a potential DNA source for postmortem human identification
Ireneusz Stołyszewski1, Anna Niemcunowicz-Janica, Witold Pepiński
1Department of Criminalistics and Forensic Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Purpose:
The aim of this study was the assessment of vitreous humor as a potential DNA for forensic human postmortem identification.
Material And Methods:
Vitreous humor samples were collected using two alternative approaches from 25 corpses of either sex during autopsies. DNA was extracted by standard organic method. Recovered DNA was quantitiated fluorometrically. AmpFlSTR SGM Plus kit and ABI 310 Genetic Analyzer (Applera) were used to obtain genetic profiles.
Results:
Different DNA yields were quantitated in vitreous body depending on cause of death and sampling approach.
Conclusion:
Vitreous humor is a potential DNA for forensic human postmortem identification depending on a sampling method used.
