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[Multicenter study on purified protein C concentrates and defined plasma levels]
V Regnault1, L Houbouyan, C Michalski
1INSERM U 284, Plateau de Brabois, Vandoeuvre-les-Nancy.
Annales De Biologie Clinique
|January 1, 1991
Summary
Performance of protein C assays varies. While calibrated plasmas showed close results across labs, high purity concentrates did not. Proper dilution buffers are crucial for accurate protein C concentrate measurement.
Area of Science:
- Clinical Chemistry
- Biotechnology
- Hematology
Background:
- Commercial protein C assays are widely available for clinical use.
- Accurate measurement of protein C is essential for therapeutic monitoring and diagnosis.
Purpose of the Study:
- To evaluate the performance and comparability of various protein C assays.
- To identify factors influencing the accuracy of protein C measurements, particularly for therapeutic concentrates.
Main Methods:
- A collaborative study involving five laboratories.
- Distribution of calibrated plasmas and high purity protein C concentrates for assay.
- Comparison of results from functional and immunological protein C assays.
Main Results:
- Protein C levels were highly consistent for calibrated plasmas across different assays and laboratories.
- Significant variability was observed in protein C measurements for high purity concentrates.
- The choice of standard and dilution buffer critically impacted the results for concentrates.
Conclusions:
- Standardized protocols for diluting protein C concentrates are necessary for accurate assay performance.
- Diluting concentrates in protein C deficient plasma improves the accuracy of functional assays by normalizing protein levels.