Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Liver Regeneration01:24

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are large...
Hepatitis01:25

Hepatitis

Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver. The...
Transducer Mechanism: Nuclear Receptors01:31

Transducer Mechanism: Nuclear Receptors

Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adulthood outcomes of patients with biliary atresia living with their native liver.

Archives de pediatrie : organe officiel de la Societe francaise de pediatrie·2026
Same author

Echocardiography, Bile Acids and Spleen Stiffness to Rule Out High-Risk Varices in Children With Biliary Atresia.

Liver international : official journal of the International Association for the Study of the Liver·2026
Same author

Molecular characterisation of the trafficking rescue of defective ABCB4 variants by roscovitine analogues.

Scientific reports·2026
Same author

Bilhemia in a Liver Transplanted Child: A Rare but Severe Complication of Percutaneous Transhepatic Cholangiography.

Pediatric transplantation·2026
Same author

Autoantibodies neutralizing type I IFNs in patients with fulminant herpes simplex virus hepatitis.

The Journal of experimental medicine·2025
Same author

Condition-Specific Growth Charts for Children With Alagille Syndrome.

JAMA network open·2025

Related Experiment Video

Updated: Jul 14, 2026

Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control
07:35

Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control

Published on: April 5, 2021

Rat hepatocytes express functional P2X receptors.

Emmanuel Gonzales1, Sylvie Prigent, Aurélie Abou-Lovergne

  • 1INSERM, Université Paris-Sud, UMR-S757, Bâtiment 443, 15 rue Georges Clémenceau, Orsay Cedex, France. emmanuel.gonzales@gmail.com

FEBS Letters
|June 29, 2007
PubMed
Summary

Rat hepatocytes express functional P2X4 and P2X7 receptors, which are ATP-gated ion channels. These findings reveal new insights into purinergic signaling in liver cells.

More Related Videos

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels
16:36

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels

Published on: May 18, 2009

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
10:25

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

Published on: February 19, 2019

Related Experiment Videos

Last Updated: Jul 14, 2026

Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control
07:35

Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control

Published on: April 5, 2021

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels
16:36

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels

Published on: May 18, 2009

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
10:25

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

Published on: February 19, 2019

Area of Science:

  • Hepatology
  • Cellular Physiology
  • Molecular Biology

Background:

  • Extracellular ATP regulates liver functions via purinergic receptors.
  • Previous studies focused only on G protein-coupled P2Y receptors in hepatocytes.

Purpose of the Study:

  • To investigate the functional expression of P2X receptors, specifically ATP-gated channels, in rat hepatocytes.
  • To determine if P2X4 and P2X7 receptors are present and active in liver cells.

Main Methods:

  • Detection of P2X4 and P2X7 transcripts and proteins using RT-PCR, Western blotting, and immunocytochemistry.
  • Assessing cellular responses like membrane blebbing and dye uptake upon stimulation with ATP and P2X7-preferring agonists.
  • Evaluating the effect of oxidized ATP, a P2X receptor blocker, on these responses.

Main Results:

  • P2X4 and P2X7 receptor transcripts and proteins were successfully detected in rat hepatocytes.
  • High concentrations of ATP and a P2X7-specific agonist induced membrane blebbing and dye uptake.
  • These ATP-induced effects were significantly inhibited by oxidized ATP, confirming P2X receptor involvement.

Conclusions:

  • P2X4 and P2X7 receptors are expressed and functionally active on rat hepatocytes.
  • These findings suggest a significant role for P2X receptors in hepatic purinergic signaling.
  • This study expands the understanding of purinergic receptor subtypes involved in liver cell function.