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Related Experiment Videos

Morphine effects on striatal transcriptome in mice.

Michal Korostynski1, Marcin Piechota, Dorota Kaminska

  • 1Department of Molecular Neuropharmacology, Institute of Pharmacology PAS, Smetna 12, 31-343, Krakow, Poland.

Genome Biology
|June 30, 2007
PubMed
Summary
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Chronic opiate use causes changes in gene expression that vary by mouse strain. Glucocorticoid receptor (GR) signaling influences morphine

Area of Science:

  • Neuroscience
  • Genomics
  • Pharmacology

Background:

  • Chronic opiate use leads to neuroadaptations, including tolerance, physical dependence, and addiction.
  • Understanding the genetic basis of these adaptations is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate genome-wide transcriptional changes induced by morphine in different inbred mouse strains.
  • To identify novel morphine-regulated genes and explore strain-specific expression patterns.
  • To determine the role of the glucocorticoid receptor (GR) in mediating behavioral responses to morphine.

Main Methods:

  • Microarray-based gene expression profiling of mouse striatum after single and repeated morphine administration.
  • Ontologic classification of morphine-responsive transcripts.

Related Experiment Videos

  • Analysis of gene expression patterns and their regulation by the glucocorticoid receptor (GR).

    • Main Results:

    • Identified 618 morphine-responsive transcripts in the striatum.
    • Discovered novel morphine-regulated genes (e.g., Olig2, Camk1g) and strain-specific expression changes (e.g., Hspa1a, Fzd2).
    • Found that GR blockade altered morphine-induced locomotor activity and physical dependence, suggesting GR involvement.

    Conclusions:

    • Significant strain-dependent differences exist in the transcriptional response to acute and chronic morphine.
    • Whole-genome analysis revealed physiological factors and gene networks influencing opioid-related phenotypes.
    • GR-regulated genes are implicated in mediating behavioral responses to morphine.