Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Considerations for Rodent Surgery10:12

Considerations for Rodent Surgery

24.0K
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN
The Guide for the Care and Use of Laboratory Animals1 dictates that rodent survival surgery be performed aseptically. Aseptic technique utilizes specific practices that minimize the contamination of the surgical site, including patient preparation, surgeon preparation, sterilization of instruments and other supplies, and the use of a clean and controlled environment. Presurgical planning,...
24.0K
In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter05:04

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter

3.8K
We describe here, the establishment and application of an Tg(Myh6-cre)1Jmk/J /Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J (referred to as αMHC-Cre/Rosa26A-Flox-Stop-Flox-GCaMP3 below) mouse reporter line for cardiac reprogramming assessment. Neonatal cardiac fibroblasts (NCFs) isolated from the mouse strain are converted into induced cardiomyocytes (iCMs), allowing for convenient and efficient evaluation of reprogramming efficiency and functional maturation of iCMs via calcium (Ca2+)...
3.8K
Noninvasive Determination of Vortex Formation Time Using Transesophageal Echocardiography During Cardiac Surgery04:48

Noninvasive Determination of Vortex Formation Time Using Transesophageal Echocardiography During Cardiac Surgery

8.3K
We describe a protocol to measure vortex formation time, an index of left ventricular filling efficiency, using standard transesophageal echocardiography techniques in patients undergoing cardiac surgery. We apply this technique to analyze vortex formation time in several groups of patients with differing cardiac...
8.3K
A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation06:57

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

9.4K
This protocol describes a net mold-based method to create three-dimensional scaffold-free cardiac tissues with satisfactory structural integrity and synchronous beating...
9.4K
Surgical Swine Model of Chronic Cardiac Ischemia Treated by Off-Pump Coronary Artery Bypass Graft Surgery09:12

Surgical Swine Model of Chronic Cardiac Ischemia Treated by Off-Pump Coronary Artery Bypass Graft Surgery

9.9K
This protocol presents a surgical large animal model of chronic, single vessel ischemia that results in regional abnormalities but does not create infarct, known as hibernating myocardium. Following establishment of chronic ischemia, animals are treated with off-pump LIMA-LAD coronary artery bypass graft surgery to revascularize the ischemic...
9.9K
Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery08:43

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

11.5K
Endothelial progenitor cells (EPCs) are crucially involved in the neovascularization of ischemic tissues. This method describes the isolation of human EPCs from peripheral blood, as well as the identification of their migratory potential against serum samples of cardiac surgical...
11.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bioprosthetic versus mechanical surgical aortic valve replacement in patients ≥65 years of age.

JTCVS open·2026
Same author

Temporizing Balloon Aortic Valvuloplasty Before Transcatheter Aortic Valve Replacement in Decompensated Severe Aortic Stenosis.

The American journal of cardiology·2026
Same author

Transcatheter vs Surgical Tricuspid Valve Repair: An Analysis of Medicare Beneficiaries.

The Annals of thoracic surgery·2026
Same author

Aortic Valve Repair for Severe Commissural Leaflet Defects Using Aortic Wall Patches.

Annals of thoracic surgery short reports·2025
Same author

Autologous leaflet reconstruction for aortic valve endocarditis.

JTCVS techniques·2025
Same author

Surgical vs Transcatheter Aortic Valve Replacement in Patients 65 Years of Age and Older.

The Annals of thoracic surgery·2025
Same journal

A CALL FOR STANDARDIZATION OF HYBRID ARCH FROZEN ELEPHANT TRUNK OUTCOMES REPORTING.

The Journal of thoracic and cardiovascular surgery·2026
Same journal

Pediatric Mitral Valve Surgery: Current Practice from the European Congenital Heart Surgeons Association Congenital Database Analysis.

The Journal of thoracic and cardiovascular surgery·2026
Same journal

Rethinking Failure to Rescue in Cardiac Surgery.

The Journal of thoracic and cardiovascular surgery·2026
Same journal

Undersized Fontan conduits are not without risk.

The Journal of thoracic and cardiovascular surgery·2026
Same journal

Predicting high-risk recipients or high-risk donation after circulatory death hearts?

The Journal of thoracic and cardiovascular surgery·2026
Same journal

Outcomes of donation after circulatory death heart transplantation in recipients with pulmonary hypertension.

The Journal of thoracic and cardiovascular surgery·2026
See all related articles

Related Experiment Video

Updated: Jan 20, 2026

Noninvasive Determination of Vortex Formation Time Using Transesophageal Echocardiography During Cardiac Surgery
04:48

Noninvasive Determination of Vortex Formation Time Using Transesophageal Echocardiography During Cardiac Surgery

Published on: November 28, 2018

8.3K

Amiodarone and cardiac surgery

J Scott Rankin

    The Journal of Thoracic and Cardiovascular Surgery
    |June 30, 2007
    PubMed
    Summary

    No abstract available in PubMed .

    More Related Videos

    Surgical Swine Model of Chronic Cardiac Ischemia Treated by Off-Pump Coronary Artery Bypass Graft Surgery
    09:12

    Surgical Swine Model of Chronic Cardiac Ischemia Treated by Off-Pump Coronary Artery Bypass Graft Surgery

    Published on: March 27, 2018

    9.9K
    Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
    08:43

    Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

    Published on: February 14, 2017

    11.5K

    Related Experiment Videos

    Last Updated: Jan 20, 2026

    Noninvasive Determination of Vortex Formation Time Using Transesophageal Echocardiography During Cardiac Surgery
    04:48

    Noninvasive Determination of Vortex Formation Time Using Transesophageal Echocardiography During Cardiac Surgery

    Published on: November 28, 2018

    8.3K
    Surgical Swine Model of Chronic Cardiac Ischemia Treated by Off-Pump Coronary Artery Bypass Graft Surgery
    09:12

    Surgical Swine Model of Chronic Cardiac Ischemia Treated by Off-Pump Coronary Artery Bypass Graft Surgery

    Published on: March 27, 2018

    9.9K
    Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
    08:43

    Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

    Published on: February 14, 2017

    11.5K