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Bacterial diversity in aphthous ulcers.

L Marchini1, M S Campos, A M Silva

  • 1Laboratory of Molecular Genetics and Genomics, University of Vale do Paraíba, São José dos Campos, São Paulo, Brazil.

Oral Microbiology and Immunology
|June 30, 2007
PubMed
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Bacterial diversity in recurrent aphthous ulcers differs significantly from healthy oral mucosa. Prevotella, a bacterial genus, was exclusively found in ulcer lesions, suggesting a role in this oral condition.

Area of Science:

  • Oral microbiology
  • Molecular diagnostics

Background:

  • Recurrent aphthous ulcers (RAUs) are common oral mucosal lesions with unknown etiology.
  • Understanding the microbial composition of RAUs is crucial for identifying potential causes.

Purpose of the Study:

  • To estimate the bacterial diversity in RAU lesions and control oral mucosa.
  • To utilize a culture-independent molecular approach for comprehensive analysis.

Main Methods:

  • DNA extraction from pooled samples of 10 healthy individuals and 10 RAU patients.
  • 16S ribosomal RNA bacterial gene amplification using polymerase chain reaction (PCR).
  • Sequencing of amplicons and comparison with the GenBank database.

Main Results:

Related Experiment Videos

  • Analysis of 535 clones revealed 95 bacterial species, including 62 novel phylotypes.
  • RAU lesions harbored 57 phylotypes (11 known species), while controls had 38 (5 known species).
  • The bacterial genus Prevotella was exclusively detected in RAU lesions, comprising 16% of lesion-derived clones.

    • Conclusions:

    • Significant divergence in microbiota between RAU lesions and control mucosa was observed.
    • The identified rich bacterial diversity offers a new avenue for qualitative and quantitative analyses of bacteria associated with RAUs.