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Published on: July 5, 2024
Baboon corpus luteum: size and sex steroid secretion throughout the luteal phase
M Yusoff Dawood1, Firyal S Khan-Dawood
1Department of Obstetrics and Gynecology, West Virginia University School of Medicine, Morgantown, West Virginia 26506-9188, USA. ydawood@hsc.wvu.edu
Objective:
To determine the size of the baboon corpus luteum (CL) and levels of plasma P, 17alpha-hydroxyprogesterone (17-OHP), and E2 in the ovarian vein draining it, the contralateral ovarian vein, and peripheral blood throughout the luteal phase of the menstrual cycle.
Design:
Prospective study.
Setting:
Academic department of obstetrics and gynecology in a US medical school.
Animal(S):
Corpora lutea from a cohort of 27 adult cycling baboons (Papio anubis).
Intervention(S):
Timed luteectomy.
Main Outcome Measure(S):
The authors weighed 166 CL and measured plasma P, 17-OHP, and E(2) in the blood samples obtained at luteectomy.
Result(S):
Early luteal phase corpora lutea weighed 189.1 +/- 12.3 mg (mean +/- SEM); their weight significantly increased to 239.4 +/- 8.4 mg at mid luteal phase and significantly declined to 188.3 +/- 14.0 mg in late luteal phase. Plasma P draining the CL (134.4 +/- 20.5 ng/mL in early, 167.4 +/- 18.7 ng/mL in mid, and 126.4 +/- 23.4 ng/mL in late luteal phase) was significantly higher than that in contralateral ovarian (11.0 +/- 1.4 ng/mL) and peripheral plasma (7.1 +/- 0.9 ng/mL). Similarly, levels of both plasma 17-OHP (10.9 +/- 1.5 to 15.9 +/- 2.4 ng/mL) and E2 (1.6 +/- 0.2 to 2.6 +/- 0.6 ng/mL) draining the CL were significantly higher than those from the contralateral ovary and peripheral blood (17-OHP, 1.1 +/- 0.2 ng/mL; E2, 0.2 +/- 0.05 ng/mL).
Conclusion(S):
Largest in mid luteal phase, the baboon CL secretes P, 17-OHP, and E2 throughout the luteal phase, with the highest levels seen in the ovarian vein draining the CL in the mid compared with the early and late luteal phases.
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