Solution structure of the BRK domains from CHD7
Mark D Allen1, Tomasz L Religa, Stefan M V Freund
1MRC Centre for Protein Engineering, Hills Road, Cambridge, CB2 2QH, UK.
Journal of Molecular Biology
|July 3, 2007
Summary
The structure of CHD7
Area of Science:
- Biochemistry
- Structural Biology
- Genetics
Background:
- CHD7 is a chromodomain helicase DNA binding domain (CHD) protein crucial for development.
- Mutations in CHD7 cause CHARGE syndrome, a complex congenital disorder.
- CHD7 contains BRK domains, a feature shared with Brahma/BRG1 helicases.
Purpose of the Study:
- To determine the NMR solution structure of the two BRK domains of CHD7.
- To provide insights into the structural characteristics of CHD7's BRK domains.
Main Methods:
- Nuclear Magnetic Resonance (NMR) spectroscopy was used to determine the structure.
- Structural analysis of the BRK domains.
Main Results:
- The two BRK domains of CHD7 adopt a compact beta beta alpha beta fold.
- The second BRK domain possesses a unique C-terminal extension with two helices.
- The determined structure of CHD7's BRK domains differs from other chromatin-associated protein domains.
Conclusions:
- The unique structure of CHD7's BRK domains may influence its function in chromatin remodeling.
- Understanding CHD7 structure is vital for CHARGE syndrome research.
- This structural information contributes to the broader understanding of CHD protein family functions.
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