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Updated: Jul 14, 2026
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
An unambiguous assignment and structural analysis using solution NMR experiments of O-antigen from Escherichia coli
Rui Tada1, Noriko Nagi-Miura, Yoshiyuki Adachi
1Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan.
Abstract:
Bacterial lipopolysaccharide from Escherichia coli O9 (O9 LPS) has various characteristic biological activities other than endotoxic activities. The biological activities exhibited depend on the structure of the O-antigen. The O-antigen region of O9 LPS is composed of the mannose homopolysaccharide (MHP). This structure was reported previously, but not all its proton and carbon signals were assigned. In the present study, we completely assign all proton and carbon signals of the O-antigen of O9 LPS using (1)H- and (13)C-NMR spectroscopy, including the DQF-COSY, TOCSY, NOESY, HSQC, H2BC, HSQC-TOCSY and HMBC methods.
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