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Published on: April 1, 2022
gp130 is important for the normal morphogenesis of Meckel's cartilage and subsequent mandibular development
Jung Won Choi1, Jung Tak Kim, Jae Han Park
1Department of Oral Pathology, School of Dentistry, Kyungpook National University, Daegu 700-412, Korea.
Abstract:
gp130-mediated signaling is involved in both chondrogenesis and osteogenesis, but its direct role in the formation of embryonic Meckel's cartilage and associated mandibular development has not yet been elucidated. In this study, we examined the influence of gp130 ablation on the developing mandibular Meckel's cartilage by evaluating the morphological and histological changes as well as the gene expression patterns in developing embryonic gp130-/- mice. The ablation of the gp130 gene showed no change in region-specific collagen mRNA expression except for a slight delay in its expression but caused shortened embryonic Meckel's cartilage, delayed hypertrophic chondrocyte maturation and subsequent bony replacement with characteristic bending of the intramandibular Meckel's cartilage. The bending of Meckel's cartilage led to a narrow mandibular arch at the rostral area with poor cortical plate formation. These findings indicate that gp130-mediated signaling is important for the normal morphogenesis of Meckel's cartilage and subsequent mandibular development.
Insights
gp130 signaling is crucial for Meckel's cartilage development. Ablating gp130 in mice resulted in shortened cartilage, delayed maturation, and mandibular arch defects, impacting bone formation.
Area of Science:
- Developmental Biology
- Molecular Biology
- Biochemistry
Background:
- gp130-mediated signaling is known to influence chondrogenesis and osteogenesis.
- Its specific role in embryonic Meckel's cartilage and mandibular development remains unclear.
Purpose of the Study:
- To investigate the function of gp130 signaling in embryonic Meckel's cartilage formation and mandibular development.
- To elucidate the effects of gp130 gene ablation on cartilage morphology, histology, and gene expression.
Main Methods:
- Utilized gp130-/- mice to study gene ablation effects.
- Evaluated morphological and histological changes in developing Meckel's cartilage.
- Analyzed gene expression patterns, focusing on collagen mRNA.
Main Results:
- gp130 gene ablation led to shortened embryonic Meckel's cartilage.
- Delayed hypertrophic chondrocyte maturation and subsequent bony replacement were observed.
- Characteristic bending of Meckel's cartilage resulted in a narrow mandibular arch and poor cortical plate formation.
- No significant changes in region-specific collagen mRNA expression, except for a slight delay.
Conclusions:
- gp130-mediated signaling is essential for the normal morphogenesis of Meckel's cartilage.
- gp130 plays a critical role in subsequent mandibular development, influencing cartilage structure and bone formation.
