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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
Published on: August 6, 2014
LambdaN-GFP: an RNA reporter system for live-cell imaging
Nathalie Daigle1, Jan Ellenberg
1Gene Expression Unit, EMBL, Meyerhofstr. 1, D-69117 Heidelberg, Germany.
Nature Methods
|July 3, 2007
Abstract:
We describe a GFP-based RNA reporter system (lambdaN-GFP) to visualize RNA molecules in live mammalian cells. It consists of GFP fused to an arginine-rich peptide derived from the phage lambda N protein, lambdaN22, which binds a unique minimal RNA motif and can be used to tag any RNA molecule. LambdaN-GFP uses a small and easy to engineer RNA tag, reducing the likelihood of perturbing the function of the tagged RNA molecule.

