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Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
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Controlled adjustment of PCR amplification

Andreas Pahl1

  • 1Department of Experimental and Clinical Pharmacology and Toxicology, Emil Fischer Center, University of Erlangen-Nuremberg, D-91054 Erlangen, Germany. pahl@pharmakologie.uni-erlangen.de

Analytical Biochemistry
|July 3, 2007
PubMed
Summary

No abstract available in PubMed .

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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