Related Experiment Videos
The expression of proteins and activities of metabolic enzymes in transplanted brain tissue
S F Leong1, T K Leung, S K Leong
1Department of Physiology, National University of Singapore, Kent Ridge.
Abstract:
The middle three-fifths of the forebrains of 14-day-old embryos were obtained and transplanted into the cortical cavities of adult rats made 7 days prior to the transplantation. The expression of proteins, as revealed by 2-dimensional gel electrophoresis studies, and the activities of energy metabolizing enzymes in the mature allografts were compared with those in the 14-day-old embryonic forebrains and corresponding areas in the contralateral cerebral hemispheres of the hosts. They were shown to approach adult pattern and adult values after 10-12 weeks of growth. The biochemical findings were discussed and correlated with some of the anatomical observations.