Related Experiment Video
Updated: May 1, 2026
Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
Published on: January 7, 2019
[Study on chromatographic fingerprint of extractive from Tripterygium wilfordii with supercritical carbon dioxide]
Wen Zhou1, Hong-ru Li, Shu-fen Li
1School of Pharmaceutical Sciences of Tianjin, Basic Medical Research Center, Tianjin Medical University, Tianjin 300070, China.
Objective:
To establish the chromatographic fingerprint of supercritical carbon dioxide extract of Tripterygium wilfordii.
Method:
HPLC method was applied for quality assessment of T. Wilfordii, HPLC analysis was performed on Kromasil C18 (4. 6 mm x 250 mm, 5 microm) with the mixture of acetonitrile-1% per thousand H3PO4, as mobile phase in gradient mode. The samples were detected at UV of 267 nm with column temperature of 35 degrees C, analytic time was 80 min; Flow-rate was 1.0 mL x min(-1). The chromatographic fingerprint of ten batches of samples was determined, for establishing the chromatographic fingerprint of T. Wilfordii.
Result:
Indicating 27 peaks in common, identified 21 peaks with chemical reference and HPLC-MS, and the HPLC fingerprint was established.
Conclusion:
The method is steady and accurate with a good repeatability and can be used as a quality control method for T. Wilfordii.
