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Purification and some properties of beta-fructofuranosidase I formed by Aureobasidium pullulans DSM 2404
Jun Yoshikawa1, Seigo Amachi, Hirofumi Shinoyama
1Department of Advanced Bioresources Science, Graduate School of Science and Technology, Chiba University, 648 Matsudo, Matsudo-City, Chiba, Japan.
Abstract:
beta-Fructofuranosidase I (FFase I) formed by Aureobasidium pullulans DSM 2404 was purified. The enzyme had a molecular weight of about 430 kDa, was not affected by various metal ions and showed high transfructosylating activity. The yield of fructooligosaccharides production using purified FFase I was 62%.
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