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Updated: Jul 13, 2026
Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging
Published on: November 24, 2021
Fluorescent silica colloids for study and visualization of skin care products
Swaminathan Iyer1, Yaroslav Kievsky, Igor Sokolov
1Department of Physics, Clarkson University, Potsdam, NY 13699-5820, USA.
Background:
The efficacy of skin care products depends on the time and dynamics of their absorbance by the skin, and its spatial distribution on the skin. Regular scrape-based methods may depend on the operator and are destructive and invasive in nature. Here, we describe a novel method based on non-contact optical measurements to trace the location and dynamics of skin care products on the skin.
Methods:
We use fluorescent silica colloidal particles of micron sizes at a rather small concentration as non-invasive tracers. As an example of skin care products, we use two base materials: either glycerin or vaseline. A mixture of each product with fluorescent particles is applied on human skin. The amount of fluorescence is monitored by means of a fluorescent spectrometer. The scraping method is used to compare with the spectroscopic measurements.
Results:
Fluorescent tracers make the skin care product visible under UV light. This allows obtaining an optical image of the spatial distribution of the product on the skin. The quantitative data of fluorescence are well correlated with the scrape data. Comparison of the difference in the spectral and scraped mass data reveals the details of accumulation of the skin products in skin cracks and crevices.
Conclusion:
We described an efficient non-invasive benign method to quantify dynamics and to perform mapping of emollients and humectants on the skin.
