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Related Experiment Video

Updated: Jul 13, 2026

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
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Simian immunodeficiency virus vector pseudotypes differ in transduction efficiency and target cell specificity in

B Liehl1, J Hlavaty, R Moldzio

  • 1Research Institute for Virology and Biomedicine, University of Veterinary Medicine, Vienna, Austria.

Gene Therapy
|July 6, 2007
PubMed
Summary

Simian immunodeficiency virus (SIV)-based lentiviral vectors efficiently transduce brain cells. VSV-G pseudotyped vectors show high efficiency and infect neuronal and glial cells, crucial for neurodegenerative disease gene therapy.

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Area of Science:

  • Neuroscience
  • Gene Therapy
  • Virology

Background:

  • Lentiviral vectors are promising for central nervous system (CNS) gene delivery.
  • Evaluating different pseudotyping glycoproteins is crucial for optimizing CNS transduction.

Purpose of the Study:

  • To assess CNS transduction efficiencies and tropism of SIV-derived lentiviral vectors with various glycoproteins.
  • To investigate the role of the central polypurine tract (cPPT) in SIV vector-mediated brain cell infection.

Main Methods:

  • SIV lentiviral vectors pseudotyped with VSV-G, MLV4070Aenv, LCMV-GP, RRV-GP, and RV-G were generated.
  • Vectors were injected stereotactically into the mouse striatum for in vivo analysis.
  • Transduction efficiency, cell tropism, cPPT influence, immune response, and biodistribution were evaluated.

Main Results:

  • VSV-G pseudotypes demonstrated high transduction efficiency, infecting both neuronal and glial cells.
  • LCMV-GP and RV-G showed moderate efficiency; MLV4070Aenv and RRV-GP showed weak efficiency.
  • Deletion of cPPT reduced in vivo transduction and abolished neuronal cell infection.
  • Humoral immune response was detected, but no gross inflammation or systemic vector spread.

Conclusions:

  • SIV vectors, particularly VSV-G pseudotyped, are efficient gene delivery vehicles for the CNS.
  • VSV-G pseudotypes offer broad tropism for neuronal and glial cells.
  • The cPPT sequence is essential for efficient neuronal transduction in vivo.
  • SIV vectors show potential for treating neurodegenerative diseases with minimal immune reaction and systemic spread.