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A single-copy promoter-cloning vector for use in Escherichia coli
1Department of Microbiology and Immunology, Emory University Health Sciences Center, Atlanta, GA 30322.
Gene
|December 1, 1991
Abstract:
We have constructed and tested a single-copy-plasmid vector (pEU720) based on the IncFII-group plasmid, R100, that is useful for cloning promoters in front of lacZ. The vector is 15 kb long and contains a unique XhoI site in front of lacZ.