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Related Experiment Video

Updated: Jul 13, 2026

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
10:31

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Published on: April 8, 2016

Reverse transcriptase activity in human normal and psoriatic skin samples.

J-P Molès1, A Tesniere, J-J Guilhou

  • 1Laboratoire de Dermatologie Moléculaire, Université Montpellier I, 641 avenue du Doyen G. Giraud, 34093 Montpellier cedex 5, France. jpm@montp.inserm.fr

The British Journal of Dermatology
|July 7, 2007
PubMed
Summary

Human endogenous retroelements express functional reverse transcriptase (RT) proteins in the skin. Psoriasis is associated with increased RT expression and activity, suggesting a role in skin pathology.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Dermatology

Background:

  • The human genome contains ancient transposable elements, some retaining coding capacity.
  • These elements, including endogenous retroelements, may play roles in physiological and pathological processes.
  • Reverse transcriptase (RT) is a key enzyme in retrotransposition.

Purpose of the Study:

  • To determine if human endogenous retroelements express functional RT proteins.
  • To investigate RT expression and activity in normal and psoriatic skin.
  • To associate RT with the pathology of psoriasis.

Main Methods:

  • Immunohistochemistry and Western blot to detect RT protein expression in skin sections and extracts.
  • Colorimetric assays to measure RT activity in normal and psoriatic skin extracts.

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  • Specific assays optimized for different retroviral types (C-type and D-type).

    • Main Results:

    • RT proteins were detected in normal skin epidermis and dermis, with a prominent 57 kDa band.
    • Psoriatic lesions showed increased RT-positive cells in both epidermal and dermal compartments, notably in Munro's abscesses.
    • RT activity was 2-3 times higher in psoriatic skin extracts compared to normal skin.

    Conclusions:

    • Functional RT proteins are expressed by active endogenous retroelements in the skin.
    • While basal RT expression is present in normal skin, activity is low.
    • Psoriasis is characterized by elevated RT-positive cells and significantly increased RT activity, potentially linked to keratinocyte hyperproliferation and inflammation.
    • Endogenous retroelements may have a significant role in skin physiology and pathology, warranting further investigation.