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Updated: Jul 13, 2026

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
Published on: July 3, 2020
Identification of osteopontin phosphorylation sites involved in bone remodeling and inhibition of pathological
Fawzy A Saad1, Erdjan Salih, Melvin J Glimcher
1Laboratory for Study of Skeletal Disorders and Rehabilitation, Department of Orthopaedic Surgery, Harvard Medical School, Children's Hospital Boston, 300 Longwood Avenue, Boston, Massachusetts 02115, USA. fawzy.saad@childrens.harvard.edu
Abstract:
Osteopontin is a noncollagenous, phosphorylated extracellular glycoprotein, expressed in mineralized and nonmineralized tissues, organs and body fluids. The protein contains an RGD tripeptide cell-binding motif, and is subjected to a variety of posttranslational modifications that play important roles in its multiple biological functions, such as bone remodeling and inhibition of pathological calcification. In this study, we have expressed bovine osteopontin in a prokaryotic system and identified the seven amino acid residues phosphorylated in vitro by CKII.
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